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Reagents and tools table

Journal: EMBO Molecular Medicine

Article Title: Ependymal cell lineage reprogramming as a potential therapeutic intervention for hydrocephalus

doi: 10.1038/s44321-024-00156-5

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Samples were incubated with primary antibodies in blocking solution at 4 °C for 24–48 h. The following primary antibodies were used: chicken anti-GFP (1020, Aves Laboratories, 1:500 for brain sections; 1:1000 for cells, 1:250 for whole mounts), mouse anti-S100β (S2532, Sigma, 1:250), rabbit anti-P73 (ab40658, Abcam, 1:300), mouse anti-FoxJ1 (14-9965, eBioscience, 1:500), rabbit anti-pericentrin (PRB432C, Covance, 1:750 for brain sections; 1:1500 for cells), rabbit anti-GFAP (Z0334, DakoCytomation, 1:1000 for cells, 1:500 for whole mounts), mouse anti-acetylated α-tubulin (T6793, Sigma, 1:1000 for brain sections; 1:1500 for cells), rabbit anti-McIdas ((Pefani et al, ), 1:250), mouse anti-centriolin (sc-365521, Santa Cruz, 1:100, cells’ fixation in PFA-Methanol), rabbit anti-Meig1 (orb185566, Biorbyt, 1:100), rat anti-VCAM1 (550547, BD Biosciences, 1:100), mouse anti β-catenin (610153, BD Biosciences, 1:500), guinea pig anti-double-cortin (AB2253, Millipore, 1:500).

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